Antiproliferative effect of GTS‑21 in glioblastoma cells

Glioblastoma multiforme (GBM) is the most common malignant brain tumour in adults. The poor prognosis and short median overall survival of patients with GBM associated resistance to therapy after surgical adjuvant treatment. expression various acetylcholine receptors (AChR) has been widely reported. present study aimed investigate cholinergic system‑related genes primary explore antiproliferative effect 3‑(2,4‑dimethoxybenzylidene) anabaseine (GTS‑21) cell lines. Therefore, 28 system was detected using a customized RT2 Profiler PCR Array 44 5 healthy control tissue samples. In addition, activity GTS‑21, an alpha 7 subunit nicotinic AChR (α7 nAChR) agonist, that α‑bungarotoxin (α‑BTX), α7 nAChR antagonist, determined established cells. A172, U87 G28 lines cells were treated ACh or nicotine. Cell viability evaluated MTT assay at 24, 48 72 h following treatment corresponding compounds. results revealed components notably downregulated, except receptor (CHRNA7), However, dominant‑negative duplicate form CHRNA7 also downregulated. Furthermore, A172 exhibited heterogeneous gene pattern. Additionally, GTS‑21 inhibited proliferation dose‑ time‑dependent manner. Interestingly, α‑BTX restored cells, but not Collectively, findings suggested may inhibit therefore serve as novel therapeutic approach GBM, which warrants further investigation.